DNA polymerases used in PCR
The following is only a partial list of products available commercially
for the PCR, and the inclusion or exclusion of any enzyme does
not indicate approval or disapproval. While we believe these facts
to be essentially correct, potential users should consult the
technical literature from the supplier of any enzyme before purchase
PE Applied Biosystems
Amplitaqrecombinant from Thermus aquataicus; t1/2 40 min at 95C; 5->3'
nuclease; 50 nt processivity
Amplitaq LDvery low bacterial DNA contamination (<4 copies of 16S RNA sequences
Amplitaq Goldbecomes active only after first high temperature cycle (due to
addition of antibody), thus suppressing products from nonspecific
primer hybridization at low temperature.
Amplitaq Stoffel fragment289 aa have been deleted from the N-terminal end, eliminating
the 5->3' nuclease activity; it is active over a wide range of
Mg++ concentration, which enables amplification from multiple
primers in the same reaction; about 2X as thermostable as the
full length polymerase.
rT threcombinant from Thermus thermophilus; has reverse transcriptase
activity (producing DNA from an RNA template) with Mn++ , as well
as DNA polymerase activity. Thus it can produce DNA copies from
RNA in one step. 20 min t 1/2 at 95C .
rT th XLa mix of rT th and the DNA polymerase from Thermococcus litoralis;
the resulting 3->5' proofreading nuclease activity and formulation
enable production of long DNA copies (>40 kb).
MJ Research as distributer for Finnzymes Oy
DyNAzyme EXTrecombinant from Thermus brockianus; t 1/2 200 min at 95C; 3->5'
proofreading nuclease activity ; can produce copies of >40 kb
New England Biolabs
Ventrecombinant from Thermococcus litoralis; t 1/2 400 min at 95C
; enzymes with and without 3->5' proofreading exonuclease activity
available; can produce copies >15 kb in length.
Deep Ventrecombinant from Pyrococcus GB-D; t 1/2 23 hours at 95C; enzymes
with and without 3->5' proofreading exonuclease activity available;
can produce copies >15 kp in length.